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The Cy0 is a new method in Real-time PCR analysis that does not require the assumption of equal efficiency between unknowns and standard curve.
The method is based on the fit of Richards' equation to real-time PCR data by nonlinear
regression in order to obtain the best fit estimators of reaction parameters. Subsequently,
these parameters were used to calculate the Cy0 value that minimizes the dependence of
its value on PCR kinetic. Our results
demonstrated that when PCR efficiency was slightly decreased, diluting
amplification mix quantity or adding a biological inhibitor such as IgG, the SCF, Ct
and Cp methods were markedly impaired while the Cy0 method gave significantly more
accurate and precise quantification results.
Cy0method.org ~
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Microbial Identification
Molzym is a manufacturer of innovative products supplying new solutions in molecular diagnostics and biology with emphasis on pre-analytical and analytical tools. Among the products are nucleic acid isolation kits and PCR/Real-Time PCR analysis reagents and assays for the detection, characterisation and manipulation of organisms.
Molzym’s proprietary technology of targeted pathogen DNA isolation, MolYsis, from complex biological systems including whole blood and other primary body fluids together with DNA-free Taq DNA polymerase, MolTaq 16S, and mastermix products, Mastermix 16S, enables the reliable 16S or 23S rDNA-based detection and monitoring of pathogens at lowest loads. These products are particularly useful for the development of PCR and hybridisation-based diagnostic systems and the study of, among others, sepsis, infections of primary body liquids, including cerebrospinal and joint fluids, periodontitis, dental caries, applied testing in pharmacological areas and environmental control.
On the basis of Molzym’s proprietary CCT technology, the PrestoSpin kits enable the high yield isolation of plasmids, genomic DNA or total RNA, respectively. Kits are available for the extraction and purification of nucleic acids from microorganisms, including bacteriophages, bacteria and fungi, cultured cells, plants, tissues, food and soil.
For daily research and developmental PCR and Real-Time PCR analysis, Molzym manufactures and supplies highly active thermostable DNA polymerases and mastermixes.
Molzym.com ~
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