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The Cy0 is a new method in Real-time PCR analysis that does not require the assumption of equal efficiency between unknowns and standard curve.
The method is based on the fit of Richards' equation to real-time PCR data by nonlinear
regression in order to obtain the best fit estimators of reaction parameters. Subsequently,
these parameters were used to calculate the Cy0 value that minimizes the dependence of
its value on PCR kinetic. Our results
demonstrated that when PCR efficiency was slightly decreased, diluting
amplification mix quantity or adding a biological inhibitor such as IgG, the SCF, Ct
and Cp methods were markedly impaired while the Cy0 method gave significantly more
accurate and precise quantification results.
Cy0method.org ~
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Eppendorf - Products
Eppendorf is a life science company that develops, produces, and distributes systems for
use in research laboratories worldwide. Its product range includes pipettes, dispensers, and
centrifuges as well as consumables such as micro test tubes and pipette tips. In addition,
Eppendorf supplies automated devices for liquid handling, ultra-low-temperature freezers,
CO2 incubators, complete equipment for DNA amplification, and instruments and systems
for cell manipulation.
Eppendorf products are aimed at academic and commercial research institutes as well
as biotechnology firms and industrial companies applying biotech research processes.
Dsdna.com ~
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Similar Sites:
gradientcycler.com
- gradientpcr.com
- insitupcr.com
- ssdna.com
- epgreen.com
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Primary Care Rheumatology Society - Primary Care Rheumatology Society
The Primary Care Rheumatology Society (PCR) was 1986 by a group of general practitioners with a special interest in Rheumatology. PCR's aims are to improve education in Rheumatology in general practice, to set up relevant research topics, to increase communication between hospital rheumatologists and other relevant health professionals, with the ultimate aim of improving care for patients with rheumatic disease.
Pcrsociety.org ~
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